A Multiparametric Live-Cell Cytotoxicity analysis using the Operetta

Cytotoxicity is a very complex process affecting multiple pathways. The ability to measure early indicators of toxicity is an essential part of drug discovery. An important approach to the detection of compound toxicity is a multiparametric analysis at the level of individual cells using High Content Analysis (HCA) applications [Abraham et al., 2008]. Such cell-based assays, which assess several cytotoxic events in a variety of relevant pathways, are considered valuable predictors of human drug toxicity. The liver, as a primary organ for drug metabolism, is the main target of many toxic effects, therefore many in vitro cellular cytotoxicity studies focus on human hepatocytes. [O`Brian et al., 2006]